Purification of tubulin and microtubules from mouse brain

Purified tubulin or microtubules are widely used for in vitro assays related to their function, such as activities of modifying enzymes and motor protein activity. This paper gave a review purification (MTs) using two different methods. One method MTs depended on polymerization depolymerization. were polymerized at 37 °C with taxol the presence. Another is purify by affinity column chromatography. Stu2p, kind MAPs, able bind C-terminal tubulin. Recombinant Stu2p was purified from expressing bacteria. Then coupled NHS-activated resins, which mouse brain. Tubulins this way can also be °C.